Results of thin-layer cytology and biopsy and the risk factors for residual and/or recurrence after loop elec-trosurgical excision procedure for cervical intraepithelial neoplastic lesions / 医学研究生学报

Objective The loop electrosurgical excision procedure (LEEP) is a major option for the treatment of cervical in-traepithelial neoplastic (CIN) lesions,but reports are rarely seen on the consistency of the result of post-LEEP pathology with that of preoperative biopsy as well as on the postoperative pathological upgrading of related factors. This study analyzes the consistency of the result of preoperative biopsy with that of post-LEEP pathology for CIN lesions and the risk factors for residual and/or recurrence after LEEP. Methods A retrospective study was conducted on 1 089 patients with CIN lesions treated by LEEP in our hospital from May 2014 to A-pril 2017. All the patients underwent liquid-based thin-layer cytology (TCT) and vaginoscopic biopsy preoperatively,followed by analysis of the results of TCT,the consistency of the result of preoperative bi-opsy with that of post-LEEP pathology,and the risk factors for residu- al and/or recurrence after LEEP. Results Preoperative biopsy showed 447 cases of low-grade squamous intraepithelial lesion (LSIL),among which TCT revealed 85 cases negative for intraepithelial lesion and malignancy (NILM),with a false negative rate of LSIL of 19.0%. Of the 645 cases of high-grade squamous intraepithelial lesion (HSIL) revealed by preoperative biopsy,TCT manifes-ted 95 cases of NILM,with a false negative rate of HSIL of 14.7%,which was significantly lower than that of LSIL (P<0.05). Preop-erative biopsy showed 447 cases of LSIL,among which post-LEEP pathology manifested 316 cases of LSIL (70.69%) and 51 cases of HSIL (11.4%). Of the 635 cases of HSIL revealed by preoperative biopsy,post-LEEP pathology exhibited 4 cases (0.63%) of inva-sive cervical cancer (ICC). Multivariate analysis showed the risk factors for pathological upgrading after LEEP were sexual partners >2 (OR=0.139,95% CI: 0.034-0.561) and LSIL in TCT (OR=0.111,95% CI: 0.040-0.310) (P<0.05) and those for postopera-tive recurrence included positive incision margin (OR=2.970,95% CI: 1.010-8.733) and persistent human papilloma virus (HPV) infection (OR=5.446,95% CI: 3.109-9.540). Preoperative HPV16 infection was correlated with lesion residual and/or recurrence after LEEP (P<0.05). Conclusion LEEP can significantly improve the diagnosis rate of HSIL in low-grade CIN patients with high risk factors. The risk factors for lesion residual and/or recurrence after LEEP include HPV16 infection,lesion involvement of the inci-sion margin,and persistent HPV infection.

High-and low-grade pancreatic neuroendocrine tumors:CT and MRI features / 天津医药

Objective To analyze the CT and MRI features of high and low grade pancreatic neuroendocrine tumors (PNET). Methods The clinical, imaging and pathological data of 32 patients with PNET collected from January 2011 to May 2017 were retrospectively analyzed.There were 21 cases with low grade PNET(G1,G2)and 11 cases with high grade PNET(G3).All of the 32 patients were performed with CT or MRI examination before operation,and pathology analysis was confirmed after operation.The imaging findings were compared between high-and low-grade PNET groups.Results In 32 patients,31 cases were with single lesion,only 1 case with two lesions.Twelve cases were in G1,9 cases in G2 and 11 cases in G3.Lesion location:10 lesions were in pancreatic head,10 lesions in body and 12 lesions in tail.Lesion shape:19 lesions were round,and 13 lesions were irregular.Lesion internal components:16 cases were solid lesion,14 cases were as a cystic-solid lesion and 2 cases were cystic lesion.Calcification was found in 5 cases.Pancreatic bile tract dilatation was found in 3 cases(both were G3).The other organs or lymph gland metastasis were found in 4 cases(both were G3),in which 2 cases were with liver metastasis, 2 cases with lymphatic metastasis. High-grade PNET was usually located in the head of the pancreas,tumors were large in size,and the shape was often irregular,showing low enhancement on enhanced CT or MRI, which can be accompanied by dilation of the pancreatic bile tract and other organs or lymph node metastasis. Conclusion High-grade and low-grade PNET shows certain imaging characteristics, such as tumor diameter, shape, location, internal components,enhancement pattern,pancreatic bile tract dilatation and metastases features,which has important value for the forecast of preoperative pathology grade of PNET.

Morphological and TLC identification on Tibetan medicine Asteris Flos / 中国中药杂志

Tibetan medicine Asteris Flos is the flowers of Aster souliei, A. flaccidus, and A. asteroides, with the function of clearing away heat and toxic matter, relieving cough, and removing phlegm. In order to control the quality of Asteris Flos, the morphological and chemical methods were established for identification of three origins. The morphological features of three species were described and photographed, and the microscopic characteristics of three drug powders were also described in detail and pictured. The results showed that three origins of Asteris Flos could be easily distinguished by their macro- and micro-morphologic features, and a key for distinguishing the three origins was given. Moreover, a TLC method, with apigenin-7-O-β-D-glucurono pyranoside and chlorogenic acid as chemical references, was also established for the identification of three origins. The results showed that the TLC chromatograms of the flowers of A. souliei and A. flaccidus were very similar, but different from that of A. asteroides. The established macroscopic, powder microscopic and TLC methods for identification of three origins of AF were simple, accurate, and reproducible, and also effective and easy to operate.

A new anthraquinone from the root of Lasianthus acuminatissimus / 药学学报

To study the constituents from the chloroform extract of the roots of Lasianthus acuminatissimus Merr., various chromatographic techniques were used to separate and purify the constituents. The structure was established on the basis of ID, 2D NMR and HRMS spectroscopic analysis. A new compound was isolated and identified, which was 3, 8-dihydroxy-1-methoxy-2-methoxymethyl-9,10-anthraquinone (I). Compound I is a new anthraquinone, namely lasianthurin.

Resource investigation and quality evaluation on wild Corydalis yanhusuo / 中国中药杂志

<p><b>OBJECTIVE</b>To study the resource and the quality of wild Corydalis yanhusuo distributed in China.</p><p><b>METHOD</b>Distribution was observed and samples of wild C. yanhusuo were collected and qualities were evaluated by determining six main alkaloids contained in the samples.</p><p><b>RESULT</b>The main distribution of wild Corydalis yanhusuo was in the hills around middle and lower reaches of Yangtze River. Its distributive areas were continuously decreasing. The alkaloids contents in the samples varied among different populations.</p><p><b>CONCLUSION</b>The alkaloids contents in wild populations of C. yanhusuo are diverse. The main kinds of alkaloids in some wild populations are higher than cultivated ones, which are valuable for breeding. The wild C. yanhusuo propagate well, however they are endangered due to environment problem, and should be protected.</p>